hsp47 floxed mice (Jackson Laboratory)
86
Structured Review
Jackson Laboratory
hsp47 floxed mice
Hsp47 Floxed Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsp47+floxed+mice/conditional+dicer+knockout+strain/pmc13144313-161-10-24
Average 86 stars, based on 1 article reviews
Hsp47 Floxed Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsp47+floxed+mice/conditional+dicer+knockout+strain/pmc13144313-161-10-24
Average 86 stars, based on 1 article reviews
hsp47 floxed mice - by Bioz Stars,
2026-10
86/100 stars
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1) Product Images from "Collagen-specific molecular chaperone Hsp47 in inguinal white adipose tissue promotes high-fat diet-induced inflammatory gene expression in male mice"
Article Title: Collagen-specific molecular chaperone Hsp47 in inguinal white adipose tissue promotes high-fat diet-induced inflammatory gene expression in male mice
Journal: Scientific Reports
doi: 10.1038/s41598-026-45003-4
Figure Legend Snippet: High-fat diet induces Hsp47 expression in adipose tissue. (a ) Immunoblot of Hsp47 and GAPDH in epiWAT (epididymal white adipose tissue) and ingWAT (inguinal white adipose tissue) from mice fed a normal diet (ND) or high-fat diet (HFD) for 12 weeks. ( b ) Quantification of Hsp47 protein (normalized to GAPDH) in epiWAT and ingWAT. ( c ) Quantification of Hsp47 mRNA (normalized to Gapdh) in epiWAT and ingWAT. ( d ) Immunoblots confirming adipose-specific Hsp47 deletion. Samples: ND flox (ND-fed Hsp47 flox/flox), ND aKO (ND-fed Adipoq-Cre; Hsp47 flox/flox), HFD flox (HFD-fed Hsp47 flox/flox), and HFD aKO (HFD-fed Adipoq-Cre; Hsp47 flox/flox). Blots shown for Hsp47, the ER chaperone Grp94, and loading controls (β-actin) in epiWAT and lung. ( e ) Quantification of Hsp47 protein level in epiWAT, ( f ) Quantification of Hsp47 protein level in lung tissue, ( g ) Quantification of Grp94 (ER stress marker) protein level in epiWAT. Data are presented as mean ± SD. Group comparisons were assessed by two-tailed t-tests. Variance equality was evaluated using the Fligner–Killeen test; when group variances were not significantly different ( P > 0.05), Student’s t-test was applied, otherwise Welch’s t-test was used. *, **, *** indicate P < 0.05, 0.01, 0.001, respectively; n.s., not significant. Exact P values are provided in Supplementary Table .
Techniques Used: Expressing, Western Blot, Marker, Two Tailed Test
Figure Legend Snippet: Adipocyte Hsp47 drives collagen deposition and adipocyte hypertrophy in inguinal white adipose tissue under a high-fat diet. (a) Representative Sirius Red–stained sections of epididymal (epiWAT) and inguinal (ingWAT) white adipose tissue from Hsp47 flox/flox (flox) and adipocyte-specific Hsp47 knockout (aKO; Adipoq-Cre; Hsp47 flox/flox) mice after 12 weeks on a normal diet (ND) or high-fat diet (HFD). Scale bars, 100 μm.
Techniques Used: Staining, Knock-Out
Figure Legend Snippet: Adipose Hsp47 knockout attenuates HFD-induced inflammatory and fibrotic gene expression in ingWAT. Relative mRNA expression of Col1a1, Col3a1, Col6a1, Fn1 (fibronectin), Il6 (interleukin-6), Tnfa (tumor necrosis factor-α), and Ccl2 (monocyte chemoattractant protein-1) in epididymal (epiWAT) and inguinal (ingWAT) white adipose tissue from mice fed a normal diet (ND) or high-fat diet (HFD) for 12 weeks, measured by qPCR (normalized to Gapdh). Data are presented as mean ± SD. Group comparisons were assessed by two-tailed t-tests. Variance equality was evaluated using the Fligner–Killeen test; when group variances were not significantly different ( P > 0.05), Student’s t-test was applied, otherwise Welch’s t-test was used. *, **, *** indicate P < 0.05, 0.01, 0.001, respectively; n.s., not significant. Exact P values are provided in Supplementary Table .
Techniques Used: Knock-Out, Gene Expression, Expressing, Two Tailed Test
Figure Legend Snippet: Adipose-specific Hsp47 knockout mitigates HFD-associated systemic and renal stress markers. Serum analytes: glucose, HDL-C (high-density lipoprotein cholesterol), inorganic phosphate (IP), calcium (Ca), and albumin; Urine analytes: urea nitrogen (U-UN), creatinine (U-CRE), uric acid (U-UA), potassium (U-K), and inorganic phosphate (U-IP). Data are presented as mean ± SD. Group comparisons were assessed by two-tailed t-tests. Variance equality was evaluated using the Fligner–Killeen test; when group variances were not significantly different ( P > 0.05), Student’s t-test was applied, otherwise Welch’s t-test was used. *, **, *** indicate P < 0.05, 0.01, 0.001, respectively; n.s., not significant. Exact P values are provided in Supplementary Table .
Techniques Used: Knock-Out, Two Tailed Test
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